Artificial Intelligence and Computational Biology in Gene Therapy: A Review.

One of the trending fields in almost all areas of science and technology is artificial intelligence. Computational biology and artificial intelligence can help gene therapy in many steps including: gene identification, gene editing, vector design, development of new macromolecules and modeling of gene delivery. There are various tools used by computational biology and artificial intelligence in this field, such as genomics, transcriptomic and proteomics data analysis, machine learning algorithms and molecular interaction studies. These tools can introduce new gene targets, novel vectors, optimized experiment conditions, predict the outcomes and suggest the best solutions to avoid undesired immune responses following gene therapy treatment.

Polymeric nanoparticles for DNA vaccine-based cancer immunotherapy: a review.

Cancer is one of the leading causes of death and mortality in the world. There is an essential need to develop new drugs or therapeutic approaches to manage treatment-resistant cancers. Cancer immunotherapy is a type of cancer treatment that uses the power of the body's immune system to prevent, control, and eliminate cancer. One of the materials used as a vaccine in immunotherapy is DNA. The application of polymeric nanoparticles as carriers for DNA vaccines could be an effective therapeutic approach to activate immune responses and increase antigen presentation efficiency. Various materials have been used as polymeric nanoparticles, including: chitosan, poly (lactic-co-glycolic acid), Polyethylenimine, dendrimers, polypeptides, and polyesters. Application of these polymer nanoparticles has several advantages, including increased vaccine delivery, enhanced antigen presentation, adjuvant effects, and more sustainable induction of the immune system. Besides many clinical trials and commercial products that were developed based on polymer nanoparticles, there is still a need for more comprehensive studies to increase the DNA vaccine efficiency in cancer immunotherapy using this type of carrier.

Exploitation of renewable energy sources for water desalination using biological tools.

The emerging impacts of climate change and the growing world population are driving the demand for more food resources and creating an urgent need for new water resources. About 93% of Earth's surface is made up of water bodies, mainly oceans. Seawater attracted a lot of attention in order to be used as a sustainable source of usable water. However, an essential step in harnessing this source of water is desalination. Utilizing renewable sources of energy, biology offers several tools for removal of salts. This article for the first time reviews all currently available biological water desalination tools and compares their efficiency with industrial systems. Bacteria are employed as electrical power generators to provide the energy needed for desalination in microbial desalination cells. Its salt removal efficiency varied from 0.8 to 30 g/L/d. Many strains of algal cells can grow in high concentrations of salts, adsorb and accumulate it inside the cell, and therefore could be used without prior treatment for seawater desalination. This biological tool can yield salt removal efficiency of 0.4-5 g/L/d. Biopolymers are also used for treatment of seawater through enhancing water evaporation as a component of solar steam generators. Despite significant advances in biological water desalination, further modifications and improvements are still needed to make its use sustainable and cost-effective.

Combinatorial biosynthesis: playing chess with the metabolism.

Secondary metabolites are a group of natural products that produced by bacteria, fungi and plants. Many applications of these compounds from medicine to industry have been discovered. However, some changes in their structure and biosynthesis mechanism are necessary for their properties to be more suitable and also for their production to be profitable. The main and most useful method to achieve this goal is combinatorial biosynthesis. This technique uses the multi-unit essence of the secondary metabolites biosynthetic enzymes to make changes in their order, structure and also the organism that produces them.

Recent advances in gene therapy: genetic bullets to the root of the problem.

Genetics and molecular genetic techniques have changed many perspectives and paradigms in medicine. Using genetic methods, many diseases have been cured or alleviated. Gene therapy, in its simplest definition, is application of genetic materials and related techniques to treat various human diseases. Evaluation of the trends in the field of medicine and therapeutics clarifies that gene therapy has attracted a lot of attention due to its powerful potential to treat a number of diseases. There are various genetic materials that can be used in gene therapy such as DNA, single- and double-stranded RNA, siRNA and shRNA. The main gene editing techniques used for in vitro and in vivo gene modification are ZNF, TALEN and CRISPR-Cas9. The latter has increased hopes for more precise and efficient gene targeting as it requires two separate recognition sites which makes it more specific and can also cause rapid and sufficient cleavage within the target sequence. There must be carriers for delivering genes to the target tissue. The most commonly used carriers for this purpose are viral vectors such as adenoviruses, adeno-associated viruses and lentiviruses. Non-viral vectors consist of bacterial vectors, liposomes, dendrimers and nanoparticles.

Recombinant Protein Purification using Composite Polyacrylamide-Nanocrystalline Cryogel Monolith Column and Carbohydrate-Binding Module Family 64 as Affinity Tag.

Background: In the field of recombinant protein production, downstream processing, especially protein purification, is critical and often the most expensive step. Carbohydrate binding module 64 (CBM64) was shown in 2011 to bind efficiently to a broad range of cellulose materials. Methods: In this study, we developed a protein purification method using nanocrystalline cellulose embedded in a polyacrylamide monolith cryogel and CBM64 affinity tag linked by intein to PD1 as a model protein. The CBM64-Intein-PD1 gene cassette was expressed in E. coli. Following cell lysis, CBM64-Intein-PD1 protein bound to the monolith PA-NCC cryogel. After washing and reducing the pH from 8.0 to 6.5, the intein underwent self-cleavage, resulting in the release and elution of pure PD1 protein. Results: The synthesized monolith column had a porous structure with an average pore size of 30 µm and a maximum binding capacity of 497 µg per gram of dried column. The yield of this purification method was 84%, while the yield of the His tag-acquired CBM64-Intein-PD1 method was 89%. Discussion: We used cellulose as support for affinity chromatography, which can be used as a cost-effective method for protein purification.

New horizons in developing cell lysis methods: A review.

Cell lysis is an essential step in many studies related to biology and medicine. Based on the scale and medium that cell lysis is carried out, there are three main types of the cell lysis: (1) lysis of the cells in the surrounding environment, (2) lysis of the isolated or cultured cells, and (3) single cell lysis. Conventionally, several cell lysis methods have been developed, such as freeze-thawing, bead beating, incursion in liquid nitrogen, sonication, and enzymatic and chemical-based approaches. In recent years, various novel technologies have been employed to develop new methods of cell lysis. The aim of studies in this field is to introduce more precise and efficient tools or to reduce the costs of cell lysis procedures. Nanostructure-based lysis methods, acoustic oscillation, electrical current, irradiation, bacteria-mediated cell lysis, magnetic ionic liquids, bacteriophage genes, monolith columns, hydraulic forces, and steam explosion are some examples of newly developed cell lysis methods. Besides the significant advances in this field, there are still many challenges and tools must be further improved.